The LRI will become part of the new Francis Crick Institute opening in 2015

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The London Research Institute research groups are based at Lincoln’s Inn Fields and Clare Hall. Our major research themes are: the biology of tumours and tissues, cellular regulatory mechanisms and genomic integrity and cell cycle.

Thomas Surrey : Microtubule Cytoskeleton

Microtubule End Tracking

 

Reconstitution of microtubule end tracking in vitro: Mal3-Alexa488 (green) localises autonomously to growing Alexa568-microtubule ends (red), visualised by TIRF microscopy. [From Bieling et al., Nature, 2007]

Tip1-GFP (green) in the presence of Tea2 and Mal3 tracks the plus end of Alexa568-microtubules (red) and moves in speckles along the microtubule lattice, visualised by TIRF microscopy. [From Bieling et al., Nature, 2007]

A N-terminal fragment of GFP-CLIP-170 (green) binds along Alexa568-microtubules (red) in the absence of EB1 (left) and tracks growing microtubule ends in the presence of EB1 (right), visualised by TIRF microscopy. [From Bieling et al., J. Cell Biol., 2008]

Cryo-EM reconstruction showing that the calponin homology domain of Mal3 binds between protofilaments of a GTPγS microtubule (a microtubule end mimic) except at the seam. The binding site is ideally positioned to sense tubulin's nucleotide state. [Modified from Maurer, Fourniol et al., Cell, 2012]